DNA Extraction Protocols for Campanulaceae

Frederick J. Peabody fpeabody at SUNFLOWR.USD.EDU
Tue Feb 18 12:20:07 CST 1997


You may try growing the plants in reduced sunlight so that the
chloroplasts are not quite so active.  Just a thought.

Frederick J. Peabody
Associate Professor of Botany
University of South Dakota
414 East Clark Street
Vermillion, SD  57069  USA
fpeabody at sundance.usd.edu

On Tue, 18 Feb 1997, W M.M. Eddie wrote:

> Can anyone suggest an improvement to the standard
> Doyle & Doyle (1987) protocol for the removal of
> polysaccharides. The Campanulaceae seem to be
> particularly bedevilled by these and although I
> have successfully extracted and amplified good
> DNA from about two dozen genera, the whole procedure
> is time-consuming and decidedly unpredictable.
> I have tried precipitation with 2.5M NaCl in addition
> to other protocols such as Dellaporta (which
> involves chloroform/phenol extraction) with only
> marginal improvement.I'm still left with a
> gelatinous mass (or mess !).
>
> Bill Eddie
>
>
>
>
> **********************************************************
>
> William M.M.Eddie
> Institute of Cell and Molecular Biology
> University of Edinburgh
> Daniel Rutherford Building
> Mayfield Road
> Edinburgh EH9 3JH
> Scotland, UK
> email: weddie at srv0.bio.ed.ac.uk
> Tel. 0131 650 5327
> FAX. 0131 650 5392
>




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