Insects for molecular work
MitchellA at NU.AC.ZA
Fri Oct 26 17:33:43 CDT 2001
It depends entirely on what you mean by "molecular work," but the bottom line is that if you want to keep your options open then an ultralow freezer (-80 C) is the only way to go.
For allozyme electrophoresis studies you will need specimens preserved at -80C. For sequencing of single-copy nuclear genes via RT-PCR (i.e. using mRNA as the template to avoid introns) the same applies, although the specimens can probably last a few weeks or even a few months preserved in 100% ethanol at room temperature.
For sequencing mitochondrial DNA and/or ribosomal DNA you can get away with room temperature ― sometimes ― for a while.
If you want to save yourself a lot of trouble wondering whether your DNA quality is okay, go with the -80C freezer. I know of a very inexpensive supplier of -80 freezers in Joburg so give me call if you want that info.
>Date: Thu, 25 Oct 2001 10:05:26 +0200
>From: Riaan Stals <VREHRS at PLANT5.AGRIC.ZA>
>Subject: Insects for molecular work
>Apologies for cross-postings.
>I ask you expert opinions, since I have been receiving contradictory
>opinions from the experts around here.
>We are about to buy a ultralow freezer for the indefinite storage of
>insects in absolute ethanol for future molecular work. The experts
>around here cannot agree whether a -30=B0C or a -70=B0C would be the
>best freezer, and this morning I received a message from another
>expert that room temperature would be just fine.
>Please enlighten me.
>VREHRS at PLANT5.AGRIC.ZA
>South African National Collection of Insects
>ARC - Plant Protection Research Institute
>Private Bag X134 Pretoria 0002 South Africa
>Voice +27-12-323-8540 Fax +27-12-325-6998
Senior Lecturer, Molecular Phylogenetics
School of Molecular and Cellular Biosciences
University of Natal
Private Bag X01
Tel: +27 (0)33 260 5815
Fax: +27 (0)33 260 6127
More information about the Taxacom