Combining insect genitalia preparation with DNA extraction

Andrew Mitchell andrew.mitchell at DPI.NSW.GOV.AU
Thu Dec 9 12:37:52 CST 2004

Hello All,

Has anyone experimented with using a proteinase-K/buffer solution to clear
insect abdomens for genitalia preparation, rather than using 10% KOH?
Seems to me this would allow you to extract DNA from the gunk that one
normally throws out...  If you have tried this yourself or know of
references please e-mail me.

Andrew Mitchell
Agricultural Scientific Collections Unit, OAI
NSW Department of Primary Industries
Forest Rd
Orange NSW 2800

Tel: +61 (0)2 6391 3848
Fax: +61 (0)2 6391 3899

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