DNA ambiguity code?

Bill McMillan mcmillan at MALA.BC.CA
Fri Nov 12 09:58:56 CST 2004


 Below is the reply I got from our resident DNA expert:

 -  Bill McMillan

****************************************

Hi Bill

My international codes does not include L either so I am also stumped as to what it refers to.  I suggest using a blast seach of the sequence and see what bases are in that position with returned sequences.

Rob

-----Original Message-----
From: Bill McMillan 
Sent: Friday, November 12, 2004 9:35 AM
To: Robert Wager
Subject: FW: DNA ambiguity code?



 Hi Rob,

 Here is the message that prompted my question.

  -  Bill

-----Original Message-----
From: Stacy J. Mantooth [mailto:stacy.mantooth at ROCKETMAIL.COM]
Sent: Thursday, November 11, 2004 1:24 PM
To: TAXACOM at LISTSERV.NHM.KU.EDU
Subject: DNA ambiguity code?


Taxacomers,

I've been tracking down some PCR primers to use in study. One primer I came across and want to use includes the letter "L" as a base in the sequence...

5' - CCTCCGTAGCCCACAYLTGYCG - 3'

I'm assuming the "L" is meant as an ambiguity code but the IUPAC code does not include the letter "L." Any ideas what this letter represents?

Thanks,

************************************************
Mr. Stacy J. Mantooth
Department of Biological Sciences
University of Nevada, Las Vegas
4505 Maryland Parkway
Las Vegas, NV 89154-4004

Office: (702) 895-2478
Fax:    (702) 895-3956

mantooth at unlv.nevada.edu
www.unlv.edu/gradasst/mantooth




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