George (Buz) Wilson
buz at MAIL.USYD.EDU.AU
Sat Mar 25 09:45:50 CST 2006
I've gotten some nice colourised SEM effects (and useful ones, too) by
capturing 3 identical images, but in three different capture modes: one in
full secondary electrons, one in full backscatter electrons, and one that
either emphasizes contrast or some other feature of the image, or just
uses a blend of backscatter and secondary electrons. Load all three images
in Photoshop and convert them to RGB colour channels. You can adjust the
appearance of the image by choosing which image goes in which colour
channel, and then modify later using the colour adjustments. You end up
with an interesting colourised image this way, but also one that has
details from all three images. I often find that converting this image
back to greyscale for normal publication brings out some features that
would be lacking in a single image capture.
Folks can contact me offline if they want to know which photoshop thingies
Don't forget that the Australian Museum is looking for two invertebrate
systematists and one terrestrial invertebrate molecular biologist. See the
Get your applications in the next week.
George (Buz) Wilson, Australian Museum
6 College Street, Sydney, NSW 2010 AUSTRALIA
EMAIL: buz.wilson at austmus.gov.au
tel: 61 2 9320 6287 fax: 61 2 9320 6042
Quoting Paulo Petry <fishnwine at CHARTER.NET>:
> You can use photoshop, transforming grayscale in rgb and them modulating
> each color chanel and adjusting hue ans saturation. I do this to false
> color x-rays and it works like a charm. In case of the x-ray, you have
> to invert the image. Thus for aech gray scale image there might be a
> different approach to render the best false coloring.
> Best Fishes,
> ---- David Orlovich <david.orlovich at BOTANY.OTAGO.AC.NZ> wrote:
> > Paul et al.
> > Year's ago I used NIH Image to put false colours on SEMs. It worked
> > by allowing the use to change the colour table for the image, so that
> > instead of going from black to white through the grey scales, it
> > would allow you to go from (say) red to blue through the colours of
> > the rainbow. You could then scroll the colour table so that it would
> > start (assign to black) any colour you want. I think that will give
> > you enough freedom to do what you want. There were different colour
> > tables to assign, so there were lots of pretty effects you could do.
> > I see that you can still get NIH Image at the following URL (it's
> > free, and it a Mac program, and also there is a Java version):
> > http://rsb.info.nih.gov/nih-image/
> > As I say, it was years ago (perhaps late 1980s - early 1990s) that I
> > was doing this, but I'm sure it is still possible with that program
> > and from memory it was easy.
> > Cheers, David.
> > On 25/03/2006, at 8:56 AM, Fabio Moretzsohn wrote:
> > > Paul
> > >
> > > Some people color monochrome (grayscale) SEM or TEM photos in
> > > Photoshop (or
> > > similar) to emphasize a certain structure of interest, or just
> > > because it
> > > looks artistic. There are different ways to colorize a black and
> > > photo, and maybe there are specialized software for this purpose.
> > >
> > > A researcher at the University of Hawaii, Tina Carvalho does some
> > > artistic colorizations of SEM and TEM micrographs colorized in
> > > Photoshop:
> > > http://www.pbrc.hawaii.edu/microangela/
> > >
> > > My two cents.
> > >
> > > Fabio
> > >
> > > -------------------------------------------------
> > > Fabio Moretzsohn, Ph.D.
> > > Post Doctoral Research Associate
> > > Harte Research Institute of Gulf of Mexico Studies
> > > Texas A&M University-Corpus Christi
> > > 6300 Ocean Drive, Unit 5869
> > > Corpus Christi, TX 78412-5869
> > > Phone: (361) 825-3230
> > > Fax: (361) 825-2050
> > > fabio at falcon.tamucc.edu
> > >
> > >
> > >
> > > ----- Original Message -----
> > > From: "Paul E. Hargraves" <pharg at GSO.URI.EDU>
> > > To: <TAXACOM at LISTSERV.NHM.KU.EDU>
> > > Sent: Friday, March 24, 2006 11:07 AM
> > > Subject: [TAXACOM] photo manipulation
> > >
> > >
> > >> Taxacomers:
> > >> Changing the recent threads abruptly, I am wrestling with a problem
> > >> I'm sure others have dealt with. One often sees former black/white
> > >> photos reproduced in various science magazines in blazing color,
> > >> SEM & TEM photos. I assume this is done with Photoshop in some
> > >> Can someone give details on how this is done?
> > >> PEH
> > >> --
> > >> Paul E. Hargraves
> > >> "Schau auf zu den Sternen, hab acht auf die Gassen" [-W. Raabe,
> > >> 1831-1910]
> > >> "Eamus, O Celtae; Eamus, O Tibialia rubentia" [anonymous, 64 B.C.]
> > Dr David Orlovich,
> > Senior Lecturer in Botany.
> > Department of Botany,
> > University of Otago,
> > P.O. Box 56,
> > (Courier: 464 Great King Street)
> > Dunedin,
> > New Zealand.
> > Phone: +643 479 9060
> > Fax: +643 479 7583
> > Mobile: +6421 122 7230
> > Web: http://www.botany.otago.ac.nz/
> > Ecology, Conservation and Biodiversity Research Group: http://
> > www.otago.ac.nz/erg/
> > Botanical Society of Otago: http://www.botany.otago.ac.nz/bso/
> > Fungal Network of New Zealand: http://www.funnz.org.nz/
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