[Taxacom] increasing depth of focus & 3D images

Wim Bert Wim.Bert at ugent.be
Tue Aug 7 02:54:53 CDT 2007

Hello image analysers,

a very cheap and simple alternative for image stacking, however without 3D 
view, is to create  "virtual microscope" movies.
see http://www.faculty.ucr.edu/~pdeley/vce.html

Video Capture and Editing microscopy is a method of recording, storing and 
modifying micrographic images as a multifocal series, by converting these 
images into multimedia video clips.

best regards,

dr. Wim BERT
Nematology Section
Department of Biology
Ghent University
Ledeganckstraat 35
9000 GENT
tel: ++32/ (0)9 264 52 23
fax: ++32/(0)9 264 53 44

----- Original Message ----- 
From: "Ethan Bright" <ethanbr at umich.edu>
To: "Jerry Cooper" <cooperj at landcareresearch.co.nz>
Cc: <taxacom at mailman.nhm.ku.edu>
Sent: Tuesday, August 07, 2007 4:02 AM
Subject: Re: [Taxacom] increasing depth of focus & 3D images

> Jerry Cooper wrote:
>> The software packages which increase depth of focus - such as Helicon,
>> CombineZ and Automontage - all use a stack of images taken at different
>> focal planes through the object. It must be possible to use this same
>> stack to create a pseudo-3D composite image that can be rotated in 3D to
>> some extent. From superficial digging I think the image analysis folks
>> even have a name for the technique - 'shape from focus'. However, I
>> can't find any software that actually does it. Photosynth is a very cool
>> piece of software but not quite what I'm after. Our image slices are
>> generated against a known frame of reference using a stepper motor
>> moving the focal plane. That extra information must surely make the task
>> simpler and more precise.
>> So, does anybody know of any software that does this?
> Try the software from MediaCybernetics
> (http://www.mediacy.com/index.aspx?page=Home), Image-Pro Express or
> Image-Pro Plus. I think it will do what you want. Same thing with
> Automontage software
> (http://www.synoptics.co.uk/syncroscopy/automontageshort.asp). Pretty
> good stuff, but unfortunately it isn't inexpensive.
> Cheers, Ethan
> -- 
> Ethan Bright
> G-139 Dana Building
> 440 Church Street
> School of Natural Resources and Environment
> The University of Michigan
> Ann Arbor, Michigan 48109-1041 USA
> Tel. 734-647-2022 (work)
> Webmaster:
> Aquatic Insects of Michigan: 
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