[Taxacom] Selective staining of suberified cells in Poaceae

Lewis, Deborah A [EEOBS] dlewis at iastate.edu
Tue May 14 11:51:43 CDT 2013


I asked Harry (Jack) Horner, who directs our microscopy and nano-imaging facility, for his suggestions. Here is his reply:
With regard to suberin staining, this is a challenge as most 'specific' stains do stain other lipid-containing substrates.  The sudan dyes stain suberin but also other substrates.

I have two alternate suggestions:
-Use sudan blue after treating sections with concentrated sulfuric acid (to remove other lipophilic substrates), washing with distilled water and then staining.  Latter treatment also may be tried with sudan IV and sudan black B.

-Also try the sulfuric acid treatment and wash; and then add 0.02 M phosphate buffer, pH 9.1, coverslip and view with UV microscope.  Suberin fluoresces yellow.

Hope this will help!
Deb Lewis

Deborah Q. Lewis, Curator
Ada Hayden Herbarium                        Phone:  [1] 515-294-9499
Iowa State University                            Email:    dlewis at iastate.edu<mailto:dlewis at iastate.edu>
340 Bessey                                                 FAX:       [1] 515-294-1337
Ames, IA  50011-1020                            Web:      www.herbarium.iastate.edu<http://www.herbarium.iastate.edu>

-----Original Message-----
From: taxacom-bounces at mailman.nhm.ku.edu [mailto:taxacom-bounces at mailman.nhm.ku.edu] On Behalf Of Martin Dubé
Sent: Monday, May 13, 2013 12:43 PM
To: taxacom at mailman.nhm.ku.edu
Subject: [Taxacom] Selective staining of suberified cells in Poaceae

For those anatomicaly oriented agrostologists,

Stains that color suberin (in so-called suberous short cells for ex.) also color other parts of tissue (chlorenchyma for ex.) making such preparations often difficult to observe, unless these latter are removed.  However, in certain cases (as with lemmas), removing the unwanted tissue is hardly possible.   I would greatly appreciate receiving suggestions from experienced botanists about a more selective stain for the suberin containing cells, i.e. not coloring cuticle or cell walls or cytoplasm.  P.S.: I have been using lactophenol cotton blue which is not selective enough.


Martin Dubé, Ph.D.
Associate professor

Campus d'Edmundston
Université de Moncton
Edmundston, NB

L'information contenue dans ce courriel et dans n'importe quelle annexe est CONFIDENTIELLE et vise uniquement la personne à qui s'adresse ce message. Toute autre distribution, copie ou divulgation est strictement interdite. Si nous vous avons transmis ce message par erreur, veuillez nous en aviser immédiatement par retour de courriel et détruire l'original ainsi que les annexes sans en faire de copie.

     Université de Moncton, campus d’Edmundston
     165, boulevard Hébert, Edmundston (N.-B.) E3V 2S8
Taxacom Mailing List
Taxacom at mailman.nhm.ku.edu<mailto:Taxacom at mailman.nhm.ku.edu>

The Taxacom Archive back to 1992 may be searched with either of these methods:

(1) by visiting http://taxacom.markmail.org

(2) a Google search specified as:  site:mailman.nhm.ku.edu/pipermail/taxacom  your search terms here

Celebrating 26 years of Taxacom in 2013.

More information about the Taxacom mailing list